Učni načrt predmeta

Predmet:
Molekularna in celična biologija lipidnih kapljic
Course:
Molecular and Cell Biology of Lipid Droplets
Študijski program in stopnja /
Study programme and level		 Študijska smer /
Study field		 Letnik /
Academic year		 Semester /
Semester
Nanoznanosti in nanotehnologije, 3. stopnja 1 1
Nanosciences and nanotechnologies, 3rd cycle 1 1
Vrsta predmeta / Course type
Izbirni / Elective
Univerzitetna koda predmeta / University course code:
NANO3-934
Predavanja
Lectures		 Seminar
Seminar		 Vaje
Tutorial		 Klinične vaje
work		 Druge oblike
študija		 Samost. delo
Individ. work		 ECTS
15 15 15 105 5

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:
izr. prof. dr. Toni Petan
Sodelavci / Lecturers:
Jeziki / Languages:
Predavanja / Lectures:
slovenščina, angleščina / Slovenian, English
Vaje / Tutorial:
Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:
Prerequisites:

Zaključen študij druge stopnje s področja naravoslovja ali tehnologije ali zaključen študij druge stopnje na drugih področjih z znanjem osnov s področja predmeta.

Completed second cycle studies in natural sciences or technologies or completed second cycle studies in other fields with knowledge of fundamentals in the field of this course.

Vsebina:
Content (Syllabus outline):

1. Uvod v lipide
Definicija in klasifikacija
Strukture in lastnosti lipidov
Vloga lipidov v bioloških membraneh in organelih

2. Presnova in signalizacija lipidov
Sinteza in razgradnja lipidov
Encimi in presnovne poti lipidov
Lipidi in signalne poti v celici

3. Osnove lipidnih kapljic
Pregled lipidnih kapljic
Struktura in funkcija lipidnih kapljic
Biosinteza in razgradnja lipidnih kapljic

4. Lipidne kapljice in membranska dinamika
Lipidne kapljice, endoplazemski retikulum in mitohondriji
Lipidne kapljice in avtofagija

1. Introduction to Lipids
Definition and classification of lipids
Chemical structure and properties of different types of lipids
Role of lipids in biological membranes and organelles

2. Lipid Metabolism and Signalling
Synthesis and degradation of lipids
Enzymes and pathways involved in lipid metabolism
Role of lipids in intracellular signaling pathways

3. Lipid Droplet Basics
Overview of lipid droplets
Structure and function of lipid droplets
Lipid droplet biogenesis and breakdown

4. Lipid Droplets and Membrane Dynamics
Lipid droplets, the endoplasmic reticulum and mitochondria
Lipid droplets and autophagy

Temeljna literatura in viri / Readings:

Basic literature:
Bosch, M., and Pol, A. (2022). Eukaryotic lipid droplets: metabolic hubs, and immune first responders. Trends Endocrinol Metab 33, 218–229.
Schott, M. B., Rozeveld, C. N., Weller, S. G., and McNiven, M. A. (2022). Lipophagy at a glance. J Cell Sci 135.
Olzmann, J. A., and Carvalho, P. (2019). Dynamics and functions of lipid droplets. Nat Rev Mol Cell Biol 20, 137–155.
Petan T (2020) Lipid droplets in cancer. In: Reviews of Physiology, Biochemistry and Pharmacology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/112_2020_51
Jarc E & Petan T (2020) A twist of FATe: Lipid droplets and inflammatory lipid mediators. Biochimie 169: 69–87
Jarc E & Petan T (2019) Lipid Droplets and the Management of Cellular Stress. Yale J Biol Med 92: 435–452
Wu H, Carvalho P & Voeltz GK (2018) Here, there, and everywhere: The importance of ER membrane contact sites. Science 361: eaan5835
Petan T, Jarc E & Jusović M (2018) Lipid Droplets in Cancer: Guardians of Fat in a Stressful World. Molecules 23: 1941
Henne WM, Reese ML & Goodman JM (2018) The assembly of lipid droplets and their roles in challenged cells. EMBO J 37: e98947
Zechner, R., Madeo, F., and Kratky, D. (2017). Cytosolic lipolysis and lipophagy: two sides of the same coin. Nat Rev Mol Cell Biol 18, 671–684.
Walther TC, Chung J & Farese RV (2017) Lipid Droplet Biogenesis. Annu Rev Cell Dev Biol 33: 491–510
Kory N, Farese RV & Walther TC (2016) Targeting Fat: Mechanisms of Protein Localization to Lipid Droplets. Trends Cell Biol 26: 535–546
Welte MA (2015) Expanding Roles for Lipid Droplets. Curr Biol 25: R470–R481
Walther TC & Jr. RVF (2012) Lipid Droplets and Cellular Lipid Metabolism. Annu Rev Biochem 81: 687–714
Greenberg, A. S., and Coleman, R. A. (2011). Expanding roles for lipid droplets. Trends Endocrinol Metab 22, 195–196.
Farese RV & Walther TC (2009) Lipid droplets finally get a little R-E-S-P-E-C-T. Cell 139: 855 860

Vsako leto bomo izbrali najbolj prebojne in odlične pregledne in raziskovalne članke na področju./Each year a selection of most up-to-date and exceptional review and research papers will be prepared.

Cilji in kompetence:
Objectives and competences:

Cilj predmeta je, da študent pridobi osnovno znanje molekularne in celične biologije lipidnih kapljic. Dobil bo najnovejša znanja o vlogi teh dinamičnih organelov v celici, o njihovih interakcijah z drugimi organeli in celičnimi procesi, ter vlogi pri razvoju bolezni. Študent se bo prav tako seznanil s specifičnimi metodološkimi pristopi za preučevanje lipidnih kapljic. Uporabljal bo najnovejšo tujo strokovno literaturo, s povdarkom na kritični analizi znanstvenih objav.

Specifične kompetence:
- študent pridobi nujno predznanje za raziskovalno delo na področju lipidov, lipidnega metabolizma in lipidnih kapljic v celičnem in molekularnobiološkem laboratoriju.

Splošne kompetence:
- razumevanje raziskovalnih metod in postopkov, razvoj kritične in samokritične presoje,
- sposobnost prenosa znanja v praksi,
- razvoj komunikacijskih sposobnosti in spretnosti, posebej komunikacije v mednarodnem okolju,
- kooperativnost, delo v skupini in tudi v mednarodnem okolju.

The goal of the course is for the student to acquire the basics of molecular and cellular biology of lipid droplets. The student will gain the latest knowledge on the role of these dynamic organelles in the cell, their interactions with other organelles and cellular processes, as well as their role in disease development. The student will also become familiar with specific methodological approaches for studying lipid droplets and will use the latest research literature, with an emphasis on critical analysis of scientific publications.

Specific competencies:
- The student will acquire the necessary background for research work in the field of lipids, lipid metabolism, and lipid droplets in a cellular and molecular biology laboratory.

General competencies:
- Understanding of research methods and procedures, development of critical and self-critical assessment,
- Ability to transfer knowledge into practice,
- Development of communication skills and proficiency, especially communication in an international environment,
- Cooperation, working in a group, and working in an international environment.

Predvideni študijski rezultati:
Intendeded learning outcomes:

Poznavanje in razumevanje osnov molekularne in celične biologije lipidov in lipidnih kapljic.
Poznavanje in razumevanje različnih raziskovalnih metod in specifičnih zahtev pri delu z lipidi in lipidnimi kapljicami.
Poznavanje in razumevanje osnov nekaterih bolezni povezanih z lipidi.

Knowledge and understanding of basic molecular and cell biology of lipids and lipid droplets.
Knowledge and understanding of different research approaches and specific requirements for lipid and lipid droplet research.
Knowledge and understanding of the hallmark characteristics of most common lipid-related diseases.

Metode poučevanja in učenja:
Learning and teaching methods:

Predavanja, konzultacije, seminar, laboratorijsko delo (opcijsko)

Lectures, consultations, seminar, laboratory work (optional)

Načini ocenjevanja:
Delež v % / Weight in %
Assesment:
Seminar
50 %
Seminar
Ustni izpit
50 %
Oral exam
Reference nosilca / Lecturer's references:
1. Petan T & Manček-Keber M (2022) Half is enough: Oxidized lysophospholipids as novel bioactive molecules. Free Radic Biol Med 188: 351–362
2. Jovičić EJ, Janež AP, Eichmann TO, Brglez V, Jordan PM, Gerstmeier J, Lambeau G, Werz O, Zimmermann R & Petan T (2021) Lipid droplets are required for lipid mediator production and cancer cell proliferation. Biorxiv: 2021.11.25.470010
3. Petan T (2020) Lipid droplets in cancer. In: Reviews of Physiology, Biochemistry and Pharmacology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/112_2020_51
4. Jarc E & Petan T (2020) A twist of FATe: Lipid droplets and inflammatory lipid mediators. Biochimie 169: 69–87
5. Jarc E, Kump A, Malavašič P, Eichmann TO, Zimmermann R & Petan T (2018) Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress. Biochim Biophys Acta - Mol Cell Biol Lipids 1863: 247–265