Učni načrt predmeta

Predmet:
Metabolomika v živilih
Course:
Foodomics
Študijski program in stopnja /
Study programme and level		 Študijska smer /
Study field		 Letnik /
Academic year		 Semester /
Semester
Ekotehnologije, 3. stopnja / 1 1
Ecotechnologies, 3rd cycle / 1 1
Vrsta predmeta / Course type
Izbirni
Univerzitetna koda predmeta / University course code:
EKO3-610
Predavanja
Lectures		 Seminar
Seminar		 Vaje
Tutorial		 Klinične vaje
work		 Druge oblike
študija		 Samost. delo
Individ. work		 ECTS
15 15 15 10 5

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:
prof. dr. Urška Vrhovšek
Sodelavci / Lecturers:
Jeziki / Languages:
Predavanja / Lectures:
slovenski, angleški / Slovene, English
Vaje / Tutorial:
Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:
Prerequisites:

Splošni pogoji za vpis na doktorski študij. studies.

General conditions for enrolment in doctoral

Vsebina:
Content (Syllabus outline):

Metabolom živil je celota spojin z nizko molekulsko maso v posameznem živilu in je lahko zelo kompleksen, saj pogosto obsega več tisoč kemijskih spojin. Živilska metabolomika se danes lahko šteje kot dragoceno orodje za ocenjevanje kakovosti, varnosti in porekla hrane ter učinkov na zdravje, povezanih s hrano. Študenti se bodo srečali z različnimi metabolomskimi pristopi v analizi različnih kemijskih skupin metabolitov hrane. Dobili bodo znanje, kako pravilno načrtovati poskus za študije metabolomike. Seznanili se bodo z različnimi tehnikami priprave vzorcev in kontrolo kakovosti primernih v metabolomskih študijah. V okviru predmeta se bodo seznanili z različnimi analitskimi tehnikami, ki se uporabljajo tako v tarčni in kot netarčni metabolomiki: tekočinsko kromatografijo, plinsko kromatografijo v povezavi z masno spektrometrijo in tudi nekaj osnovnih informacij o principu NMR metabolomike. Študenti bodo spoznali osnove metodologije podatkovnega rudarjenja kompleksnih podatkovnih nizov in pridobili znanje, kako iz njih pridobiti biološko pomembne informacije.

The food metabolome - i.e. the entirety of all low molecular weight compounds in a specific food. It can be extremely complex, often comprising several thousand chemical species. Food metabolomics can nowadays be considered as a valuable tool for the assessment of food quality, food safety, food tracability and food-related health effects. Students will meet with different metabolomic approaches in the analysis of diverse classes of food metabolites. They will get a knowledge how to properly design an experiment for metabolomics studies. They will learn about different sample preparation techniques, and quality control for metabolomics studies. In the frame of the course, several analytical technologies applied in the untargeted and targeted metabolomics studies: liquid chromatography, gas chromatography coupled to mass spectrometry and also some basic information about NMR based metabolomics will be introduced. The students will learn the basics about the data mining methodologies of a complex data sets and the knowledge how to extract biologically meaningful information.

Temeljna literatura in viri / Readings:

Saito K, Dixon RA, Willmitzer L. 2006 Plant Metabolomics: 57 (Biotechnology in Agriculture and Forestry) Kindle Edition, Springer, 347 p.
Villas-Bôas SG, Roessner U., Hansen MAE, Smedsgaard J, Nielsen J (2007) Metabolome Analysis: An Introduction, John Wiley & Sons, Inc., 311 p.
Weckwerth, W. 2007 Metabolomics: Methods in Molecular Biology 2007, Humana press, 293 p.
Robert D. Hall. 2011. Annual Plant Reviews, Volume 43, Biology of Plant Metabolomics, 420 p.
Pregledni članki iz revije Metabolomics.

Cilji in kompetence:
Objectives and competences:

Cilj tega predmeta je dati študentom znanje, kako izvesti metabolomsko študijo od začetka do konca.

Študent pridobi kompetence potebne za načrt eksperimentalnega poskusa, pripravo vzorcev, uporabo masne spektrometrije za globalno profiliranje (netarčna metabolomika) in ciljno usmerjene metodologije (tarčna metabolomika) ter znanja o analizi podatkov.

This course aims to give to the students a knowledge how to perform a metabolomic experiment, from start to finish.

The competences covers study design, sample preparation, the use of mass spectrometry for global profiling and targeted methodologies and data analysis.

Predvideni študijski rezultati:
Intendeded learning outcomes:

Predmet usposobi študenta za izvedbo celotnega poskusa, ki temelji na metabolomskem pristopu.

This course prepares the student to carry out the entire experiment, based on the metabolomic approach.

Metode poučevanja in učenja:
Learning and teaching methods:

Predavanja, priprava seminarjev - timsko delo in debate.

Lectures, seminars – team work and discussions.

Načini ocenjevanja:
Delež v % / Weight in %
Assesment:
Seminar
50 %
Seminar
Ustni zagovor seminarske naloge
50 %
Oral defence of seminar work
Reference nosilca / Lecturer's references:
1. Beckner Whitener, M.E., Stanstrup, J., Panzeri, V., Carlin, S., Divol, B., Du Toit, M., Vrhovsek, U. Untangling the wine metabolome by combining untargeted SPME–GCxGC-TOF-MS and sensory analysis to profile Sauvignon blanc co-fermented with seven different yeasts (2016) Metabolomics, 12 (3), art. no. 53, pp. 1-25.
2. Fontana, A., Copetti, M., Di Gangi, I.M., Mazza, T., Tavano, F., Gioffreda, D., Mattivi, F., Andriulli, A., Vrhovsek, U., Pazienza, V. Development of a metabolites risk score for one-year mortality risk prediction in pancreatic adenocarcinoma patients (2016) Oncotarget, 7 (8), pp. 8968-8978.
3. Gasperotti, M., Masuero, D., Guella, G., Mattivi, F., Vrhovsek, U. Development of a targeted method for twenty-three metabolites related to polyphenol gut microbial metabolism in biological samples, using SPE and UHPLC-ESI-MS/MS (2014) Talanta, 128, pp. 221-230.
4. Vrhovsek, U., Lotti, C., Masuero, D., Carlin, S., Weingart, G., Mattivi, F. Quantitative metabolic profiling of grape, apple and raspberry volatile compounds (VOCs) using a GC/MS/MS method (2014) Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 966, pp. 132-139.
5. Vrhovsek, U., Masuero, D., Gasperotti, M., Franceschi, P., Caputi, L., Viola, R., Mattivi, F. A versatile targeted metabolomics method for the rapid quantification of multiple classes of phenolics in fruits and beverages (2012) Journal of Agricultural and Food Chemistry, 60 (36), pp. 8831-8840.