Učni načrt predmeta

Predmet:

Proteinski toksini - karakterizacija in uporaba v celični biologiji

Course:

Protein Toxins -Characterisations and Applications in Cellular Biology

Študijski program in stopnja / Study programme and level	Študijska smer / Study field	Letnik / Academic year	Semester / Semester
Nanoznanosti in nanotehnologije, 3. stopnja	Bioznanosti
1 1

Vrsta predmeta / Course type

Izbirni

Univerzitetna koda predmeta / University course code:

NANO3-824

Predavanja Lectures	Seminar Seminar	Vaje Tutorial	Klinične vaje work	Druge oblike študija	Samost. delo Individ. work	ECTS
30	30			30	21	10

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:

prof. dr. Igor Križaj

Sodelavci / Lecturers:

Jeziki / Languages:

Predavanja / Lectures:

Vaje / Tutorial:

Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:

Prerequisits:

Vsebina:

Content (Syllabus outline):

Temeljna literatura in viri / Readings:

Zapiski predavanj / Lecture notes

Izbrani pregledni članki npr.: / Selected review papers e.g.:
Clark, G.C., Casewell, N.R., Elliott, C.T., Harvey, A.L., Jamieson, A.G., Strong, P.N. and Turner, A.D. (2019): Friends or Foes? Emerging Impacts of Biological Toxins. Trends Biochem Sci. 44(4): 365–379.
Rigoni, M. and Montecucco, C. (2017): Animal models for studying motor axon terminal paralysis and recovery (Review). J. Neurochem. 142 (Suppl. 2): 122–129. 
Gutiérrez, J.M., Calvete, J.J., Habib, A.G., Harrison, R.A., Williams, D.J. and Warrell, D.A. (2017): Snakebite envenoming. Nat. Rev. Dis. Primers 3: 17063.
Pirazzini, M., Rossetto, O., Eleopra, R. and Montecucco, C. (2017): Botulinum Neurotoxins:
Biology, Pharmacology, and Toxicology. Pharmacol Rev. 69(2): 200–235.
Calvete, J.J. (2017): Venomics: integrative venom proteomics and beyond. Biochem. J. 
474(5): 611–634.
Harvey, A.L. (2014): Toxins and drug discovery. Toxicon 92: 193–200.
Šribar, J., Oberčkal, J. and Križaj, I. (2014): Understanding the molecular mechanism underlying the presynaptic toxicity of secreted phospholipases A2: An update. Toxicon 89, 9–16.
Casewell, N.R., Wüster, W., Vonk, F.J., Harrison, R.A. and Fry, B.G. (2013): Complex cocktails: the evolutionary novelty of venoms. Trends Ecol. Evol. 28, 219–229.
Sajevic, T., Leonardi, A. and Križaj, I. (2011): Haemostatically active proteins in snake venoms. Toxicon 57, 627–645.

Izbrani zborniki in knjige npr.: / Selected collections and books e.g.:
Gopalakrishnakone, P., Inagaki, H., Vogel, C.-W., Mukherjee, A.K. and Rahmy, T.R. (eds.) 2017: Snake Venoms, Springer Science+Busines Media, Dordrecht.
Stöcklin, R. and Harvey, A.L. (eds.) 2012: From venoms to drugs. Toxicon 59 (4) Elsevier Ltd.

Cilji in kompetence:

Objectives and competences:

To inform the students about:
- recent experimental methods and techniques for purification of protein toxins,
- recent experimental methods and techniques for biochemical and patho-physiological characterization of protein toxins,
- selected examples of application of protein toxins as research tools,
- selected examples of the use of protein toxins in medical applications.

General Competences:
- The student will learn about the methods and procedures to isolate protein toxins and to characterize them,
- will develop the skill to critically asses his research activities,
- will develop the skill to transfer ideas from the basic knowledge pool into applications,
- will develop the skill to communicate scientific results to broader community with particular emphasis on communication in the international environment,
- will learn to work in a team (and in international environment).

Course Specific Competences:
The student will learn to pick an appropriate protein toxin to use it as a tool in his scientific work.

Predvideni študijski rezultati:

Intendeded learning outcomes:

Metode poučevanja in učenja:

Learning and teaching methods:

Načini ocenjevanja:

Delež v % / Weight in %

Assesment:

Seminar

50 %

Seminar

Ustni izpit

50 %

Oral exam

Reference nosilca / Lecturer's references:

1.	Požek, K., Leonardi, A., Pungerčar, J., Rao, W., Gao, Z., Liu, S., Laustsen, A.H., Trampuš Bakija, A., Reberšek, K., Podgornik, H. and Križaj, I. (2022): Genomic confirmation of the P-IIIe subclass of snake venom metalloproteinases and characterization of its first member, a disintegrin-like/cysteine-rich protein. Toxins 14(4), 232.
2.	Lomonte B. and Križaj, I. (2021): Snake venom phospholipase A2 toxins. Handbook of venoms and toxins of reptiles, 2nd Ed. (Stephen P. Mackessy, Ed.), CRC Press, Taylor & Francis Group, Boca Raton, Florida, USA. pp. 389–411..
3.	Leonardi, A., Sajevic, T., Pungerčar, J. and Križaj, I. (2019): A comprehensive study of the proteome and transcriptome of the venom of the most venomous European viper: Discovery of a new subclass of ancestral snake venom metalloproteinase precursor-derived proteins. J. Proteome Res. 18(5), 2287–2309.
4.	Šribar, J., Kovačič, L., Oberčkal, J., Ivanušec, A., Petan, T., Fox, J.W. and Križaj, I. (2019): The neurotoxic secreted phospholipase A2 from the Vipera a. ammodytes venom targets cytochrome c oxidase in neuronal mitochondria Sci. Rep. 9(1), 293.
5.	Latinović, Z., Leonardi, A., Kovačič, L., Koh, C.Y., Šribar, J., Trampuš Bakija, A., Venkateswarlu D., Kini, R.M. and Križaj, I. (2018): The first intrinsic tenase complex inhibitor with serine protease structure offers a new perspective in anticoagulant therapy. Thromb. Haemost. 118(10), 1713–1728.